hrp substrate Search Results


3 3 diaminobenzidine  (Vector Laboratories)
96
Vector Laboratories 3 3 diaminobenzidine
3 3 Diaminobenzidine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+substrate/DAB+Peroxidase+(HRP)+Substrate+Kit+(with+Nickel)%2C+3%2C3%E2%80%99-diaminobenzidine/pm41922744-381-14-15
Average 96 stars, based on 1 article reviews
3 3 diaminobenzidine - by Bioz Stars, 2026-09
96/100 stars
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tmb substrate  (Surmodics IVD)
95
Surmodics IVD tmb substrate
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Tmb Substrate, supplied by Surmodics IVD, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+substrate/TMB+One+Component+HRP+Microwell+Substrate/pmc13106428-247-4-6
Average 95 stars, based on 1 article reviews
tmb substrate - by Bioz Stars, 2026-09
95/100 stars
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tmb 1 component hrp microwell substrate  (ImmunoChemistry Technologies)
93
ImmunoChemistry Technologies tmb 1 component hrp microwell substrate
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Tmb 1 Component Hrp Microwell Substrate, supplied by ImmunoChemistry Technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+substrate/TMB+1-Component+HRP+Microwell+Substrate/pmc12006086-60-3-8
Average 93 stars, based on 1 article reviews
tmb 1 component hrp microwell substrate - by Bioz Stars, 2026-09
93/100 stars
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dab substrate  (Vector Laboratories)
96
Vector Laboratories dab substrate
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Dab Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+substrate/ImmPACT+DAB+Peroxidase+(HRP)+Substrate/pmc12992261-76-29-32
Average 96 stars, based on 1 article reviews
dab substrate - by Bioz Stars, 2026-09
96/100 stars
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immun star horseradish peroxidase substrate kit  (Bio-Rad)
96
Bio-Rad immun star horseradish peroxidase substrate kit
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Immun Star Horseradish Peroxidase Substrate Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+substrate/HRP+Substrate+Kit/10__1128_slash_jvi__02496___06-65-29-34
Average 96 stars, based on 1 article reviews
immun star horseradish peroxidase substrate kit - by Bioz Stars, 2026-09
96/100 stars
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colorimetric peroxide enzyme immunoassay substrate  (Bio-Rad)
95
Bio-Rad colorimetric peroxide enzyme immunoassay substrate
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Colorimetric Peroxide Enzyme Immunoassay Substrate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+substrate/HRP+Enzyme+Substrate+(TMB)/pmc02542413-204-15-21
Average 95 stars, based on 1 article reviews
colorimetric peroxide enzyme immunoassay substrate - by Bioz Stars, 2026-09
95/100 stars
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hrp substrate  (Rockland Immunochemicals)
93
Rockland Immunochemicals hrp substrate
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Hrp Substrate, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+substrate/Chemiluminescent+FemtoMax+Super+Sensitive+HRP+Substrate/pmc05919676-272-5-7
Average 93 stars, based on 1 article reviews
hrp substrate - by Bioz Stars, 2026-09
93/100 stars
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nova red kit  (Vector Laboratories)
96
Vector Laboratories nova red kit
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Nova Red Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+substrate/VECTOR+NovaRED+Peroxidase+(HRP)+Substrate+Kit/pm42036010-159-5-8
Average 96 stars, based on 1 article reviews
nova red kit - by Bioz Stars, 2026-09
96/100 stars
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hrp conjugate substrate kit  (Bio-Rad)
96
Bio-Rad hrp conjugate substrate kit
Fig. 2. SDS/PAGE and Western blotting of ECD6H expressed from Sf21 cells. Purified by concanavalin A and Ni-chelating affinity chromatography, and visualized by (A) silver stain, (B) a His6-tagged protein detection kit and (C) <t>concanavalin</t> <t>A-conjugated</t> <t>HRP.</t> PGNase F (+) and (±) indicate treatment without or with PNGase F. (D) ECD6H was treated without (lanes 1, 2) or with (lanes 3, 4) PNGase F and subsequently photoaffinity-labeled with BB-[125I-Tyr4]STp(4±17) in the absence (lanes 1, 3) and presence (lanes 2, 4) of 1026 m STp(4±17).
Hrp Conjugate Substrate Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+substrate/HRP+Conjugate+Substrate+Kit/pm10406940-64-19-23
Average 96 stars, based on 1 article reviews
hrp conjugate substrate kit - by Bioz Stars, 2026-09
96/100 stars
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one component chemiluminescent cl substrate  (Rockland Immunochemicals)
92
Rockland Immunochemicals one component chemiluminescent cl substrate
a Variation in chemiluminescence intensity with HRP concentration for liquid <t>chemiluminescent</t> substrate and lyophilized and serum-reconstituted chemiluminescent substrate and b Contact angle analysis of DI water and artificial serum on flat COC chip, dip-coated in 1% BSA in PBS for different periods of time
One Component Chemiluminescent Cl Substrate, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+substrate/UniGlow+-+One+Component+Chemiluminescent+Substrate/pmc08433401-305-2-7
Average 92 stars, based on 1 article reviews
one component chemiluminescent cl substrate - by Bioz Stars, 2026-09
92/100 stars
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aec peroxidase substrate sk 4200 kits  (Vector Laboratories)
96
Vector Laboratories aec peroxidase substrate sk 4200 kits
a Variation in chemiluminescence intensity with HRP concentration for liquid <t>chemiluminescent</t> substrate and lyophilized and serum-reconstituted chemiluminescent substrate and b Contact angle analysis of DI water and artificial serum on flat COC chip, dip-coated in 1% BSA in PBS for different periods of time
Aec Peroxidase Substrate Sk 4200 Kits, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+substrate/AEC+Peroxidase+(HRP)+Substrate+Kit%2C+3-amino-9-ethylcarbazole/us12611444-500-9-18
Average 96 stars, based on 1 article reviews
aec peroxidase substrate sk 4200 kits - by Bioz Stars, 2026-09
96/100 stars
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femtomax 110 reagents  (Rockland Immunochemicals)
93
Rockland Immunochemicals femtomax 110 reagents
a Variation in chemiluminescence intensity with HRP concentration for liquid <t>chemiluminescent</t> substrate and lyophilized and serum-reconstituted chemiluminescent substrate and b Contact angle analysis of DI water and artificial serum on flat COC chip, dip-coated in 1% BSA in PBS for different periods of time
Femtomax 110 Reagents, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+substrate/FEMTOMAX+SUPER+SENSITIVE+HRP+SUBSTRATE/pmc03777936-60-4-6
Average 93 stars, based on 1 article reviews
femtomax 110 reagents - by Bioz Stars, 2026-09
93/100 stars
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Image Search Results


CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off with PBST. Captured biotinylated mTNFα was detected with streptavidin-HRP and TMB substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.

Journal: Frontiers in Immunology

Article Title: Immunization with detoxified TNFα elicits neutralizing antibodies and ameliorates inflammatory shock and autoimmune arthritis in mice

doi: 10.3389/fimmu.2026.1801223

Figure Lengend Snippet: CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off with PBST. Captured biotinylated mTNFα was detected with streptavidin-HRP and TMB substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.

Article Snippet: After washing with PBST, TMB substrate (Surmodics, Cat# TMBW-1000-01) was added.

Techniques: Blocking Assay, Binding Assay, Vaccines, Enzyme-linked Immunosorbent Assay, Inhibition, Incubation

Fig. 2. SDS/PAGE and Western blotting of ECD6H expressed from Sf21 cells. Purified by concanavalin A and Ni-chelating affinity chromatography, and visualized by (A) silver stain, (B) a His6-tagged protein detection kit and (C) concanavalin A-conjugated HRP. PGNase F (+) and (±) indicate treatment without or with PNGase F. (D) ECD6H was treated without (lanes 1, 2) or with (lanes 3, 4) PNGase F and subsequently photoaffinity-labeled with BB-[125I-Tyr4]STp(4±17) in the absence (lanes 1, 3) and presence (lanes 2, 4) of 1026 m STp(4±17).

Journal: European journal of biochemistry

Article Title: The relevance of N-linked glycosylation to the binding of a ligand to guanylate cyclase C.

doi: 10.1046/j.1432-1327.1999.00488.x

Figure Lengend Snippet: Fig. 2. SDS/PAGE and Western blotting of ECD6H expressed from Sf21 cells. Purified by concanavalin A and Ni-chelating affinity chromatography, and visualized by (A) silver stain, (B) a His6-tagged protein detection kit and (C) concanavalin A-conjugated HRP. PGNase F (+) and (±) indicate treatment without or with PNGase F. (D) ECD6H was treated without (lanes 1, 2) or with (lanes 3, 4) PNGase F and subsequently photoaffinity-labeled with BB-[125I-Tyr4]STp(4±17) in the absence (lanes 1, 3) and presence (lanes 2, 4) of 1026 m STp(4±17).

Article Snippet: The deglycosylation of ECD6H was confirmed using succinylatedconcanavalin A conjugated with horseradish peroxidase (HRP; Vector Lab. Inc.) and an HRP Conjugate Substrate Kit (BioRad).

Techniques: SDS Page, Western Blot, Purification, Affinity Chromatography, Silver Staining, Labeling

a Variation in chemiluminescence intensity with HRP concentration for liquid chemiluminescent substrate and lyophilized and serum-reconstituted chemiluminescent substrate and b Contact angle analysis of DI water and artificial serum on flat COC chip, dip-coated in 1% BSA in PBS for different periods of time

Journal: Microsystems & Nanoengineering

Article Title: A new microchannel capillary flow assay (MCFA) platform with lyophilized chemiluminescence reagents for a smartphone-based POCT detecting malaria

doi: 10.1038/s41378-019-0108-8

Figure Lengend Snippet: a Variation in chemiluminescence intensity with HRP concentration for liquid chemiluminescent substrate and lyophilized and serum-reconstituted chemiluminescent substrate and b Contact angle analysis of DI water and artificial serum on flat COC chip, dip-coated in 1% BSA in PBS for different periods of time

Article Snippet: Uniglow TM one-component chemiluminescent (CL) substrate, Uniglow-0100 (Rockland antibodies and assay, USA) and QuantaRed TM Enhanced Chemifluorescent (CF) HRP Substrate, 15159 (Thermo Fisher Scientific), were used to perform the CL-based and CF-based assays, respectively.

Techniques: Concentration Assay

a Flowchart depicting the architecture of the android application, b Application screenshots showing step by step operation of the smartphone analyzer, c The voltage vs time plot for different concentrations of malaria biomarker PfHRP2 as recorded by the smartphone and d the voltage variation with different concentrations of malaria biomarker PfHRP2 as recorded by the smartphone analyzer is plotted (red line) as compared to the chemiluminescent intensity recorded by a conventional reader for the same PfHRP2 concentrations

Journal: Microsystems & Nanoengineering

Article Title: A new microchannel capillary flow assay (MCFA) platform with lyophilized chemiluminescence reagents for a smartphone-based POCT detecting malaria

doi: 10.1038/s41378-019-0108-8

Figure Lengend Snippet: a Flowchart depicting the architecture of the android application, b Application screenshots showing step by step operation of the smartphone analyzer, c The voltage vs time plot for different concentrations of malaria biomarker PfHRP2 as recorded by the smartphone and d the voltage variation with different concentrations of malaria biomarker PfHRP2 as recorded by the smartphone analyzer is plotted (red line) as compared to the chemiluminescent intensity recorded by a conventional reader for the same PfHRP2 concentrations

Article Snippet: Uniglow TM one-component chemiluminescent (CL) substrate, Uniglow-0100 (Rockland antibodies and assay, USA) and QuantaRed TM Enhanced Chemifluorescent (CF) HRP Substrate, 15159 (Thermo Fisher Scientific), were used to perform the CL-based and CF-based assays, respectively.

Techniques: Biomarker Discovery